Construct: shRNA TRCN0000087218
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- XM_485445.1-1056s1c1
- DNA Barcode:
- GCTCTGAGAACATCCTAGGAA
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- LOC433758 (433758)
- Gene Description:
- similar to CUB and Sushi multiple domains 2
- Transcript:
- RefSeq XM_485445.1 (NON-CURRENT)
- Match location:
- Position 1056 (3UTR)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000087218)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GCTCTGAGAACATCCTAGGAA
- Hairpin Sequence:
-
5'-CCGG-GCTCTGAGAACATCCTAGGAA-CTCGAG-TTCCTAGGATGTTCTCAGAGC-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGCTCTGAGAACATCCTAGGAACTCGAGTTCCTAGGATGTTCTCAGAGCTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGCTCTGAGAACATCCTAGGAACTCGAGTTCCTAGGATGTTCTCAGAGC-3'
Other clones with same target sequence:
(none)
