Construct: shRNA TRCN0000087222

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_485445.1-1052s1c1
DNA Barcode:
CAGAGCTCTGAGAACATCCTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
LOC433758 (433758)
Gene Description:
similar to CUB and Sushi multiple domains 2
Transcript:
RefSeq XM_485445.1 (NON-CURRENT)
Match location:
Position 1052 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000087222)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGAGCTCTGAGAACATCCTA
Hairpin Sequence:
5'-CCGG-CAGAGCTCTGAGAACATCCTA-CTCGAG-TAGGATGTTCTCAGAGCTCTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGAGCTCTGAGAACATCCTACTCGAGTAGGATGTTCTCAGAGCTCTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGAGCTCTGAGAACATCCTACTCGAGTAGGATGTTCTCAGAGCTCTG-3'

Other clones with same target sequence:

(none)