Construct: shRNA TRCN0000087396

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020626.1-391s1c1
DNA Barcode:
AGTCGGCCATAAGAAAGAATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tmem27 (57394)
Gene Description:
transmembrane protein 27
Transcript:
RefSeq NM_020626.1 (NON-CURRENT)
Match location:
Position 391 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000087396)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGTCGGCCATAAGAAAGAATA
Hairpin Sequence:
5'-CCGG-AGTCGGCCATAAGAAAGAATA-CTCGAG-TATTCTTTCTTATGGCCGACT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGTCGGCCATAAGAAAGAATACTCGAGTATTCTTTCTTATGGCCGACTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGTCGGCCATAAGAAAGAATACTCGAGTATTCTTTCTTATGGCCGACT-3'

Other clones with same target sequence:

(none)