Construct: shRNA TRCN0000087399

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_485956.1-790s1c1
DNA Barcode:
GCCTACTTCCTTCCTGAGTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
9230119C12Rik (77909)
Gene Description:
RIKEN cDNA 9230119C12 gene
Transcript:
RefSeq XM_485956.1 (NON-CURRENT)
Match location:
Position 790 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000087399)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTACTTCCTTCCTGAGTTT
Hairpin Sequence:
5'-CCGG-GCCTACTTCCTTCCTGAGTTT-CTCGAG-AAACTCAGGAAGGAAGTAGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTACTTCCTTCCTGAGTTTCTCGAGAAACTCAGGAAGGAAGTAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTACTTCCTTCCTGAGTTTCTCGAGAAACTCAGGAAGGAAGTAGGC-3'

Other clones with same target sequence:

(none)