Construct: shRNA TRCN0000087401

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_485956.1-666s1c1
DNA Barcode:
TGTGAGGGAGACTCCTATGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
9230119C12Rik (77909)
Gene Description:
RIKEN cDNA 9230119C12 gene
Transcript:
RefSeq XM_485956.1 (NON-CURRENT)
Match location:
Position 666 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000087401)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGTGAGGGAGACTCCTATGAT
Hairpin Sequence:
5'-CCGG-TGTGAGGGAGACTCCTATGAT-CTCGAG-ATCATAGGAGTCTCCCTCACA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGTGAGGGAGACTCCTATGATCTCGAGATCATAGGAGTCTCCCTCACATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGTGAGGGAGACTCCTATGATCTCGAGATCATAGGAGTCTCCCTCACA-3'

Other clones with same target sequence:

(none)