Construct: shRNA TRCN0000087415

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_147971.3-427s1c1
DNA Barcode:
GCCATGTATGTACACGCCTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
LOC224147 (224147)
Gene Description:
similar to Proteasome subunit alpha type 3 (Proteasome component C8) (Macropain subunit C8) (Multicatalytic endopeptidase complex subunit C8) (Proteasome subunit K)
Transcript:
RefSeq XM_147971.3 (NON-CURRENT)
Match location:
Position 427 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000087415)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCATGTATGTACACGCCTAT
Hairpin Sequence:
5'-CCGG-GCCATGTATGTACACGCCTAT-CTCGAG-ATAGGCGTGTACATACATGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCATGTATGTACACGCCTATCTCGAGATAGGCGTGTACATACATGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCATGTATGTACACGCCTATCTCGAGATAGGCGTGTACATACATGGC-3'

Other clones with same target sequence:

(none)