Construct: shRNA TRCN0000087452

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_487862.1-135s1c1
DNA Barcode:
AGTCAATATGAGCAGTTTGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
LOC435839 (435839)
Gene Description:
similar to ubiquitin-conjugating enzyme E2 variant 1
Transcript:
RefSeq XM_487862.1 (NON-CURRENT)
Match location:
Position 135 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000087452)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGTCAATATGAGCAGTTTGAA
Hairpin Sequence:
5'-CCGG-AGTCAATATGAGCAGTTTGAA-CTCGAG-TTCAAACTGCTCATATTGACT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGTCAATATGAGCAGTTTGAACTCGAGTTCAAACTGCTCATATTGACTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGTCAATATGAGCAGTTTGAACTCGAGTTCAAACTGCTCATATTGACT-3'

Other clones with same target sequence:

(none)