Construct: shRNA TRCN0000087454
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- XM_358993.3-592s1c1
- DNA Barcode:
- GCTGCAAAGACAGAGATAGAA
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Gm5406 (385905)
- Gene Description:
- predicted gene 5406; proteasome (prosome, macropain) subunit, alpha type 3 pseudogene
- Transcript:
- RefSeq XM_358993.3 (NON-CURRENT)
- Match location:
- Position 592 (CDS)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000087454)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GCTGCAAAGACAGAGATAGAA
- Hairpin Sequence:
-
5'-CCGG-GCTGCAAAGACAGAGATAGAA-CTCGAG-TTCTATCTCTGTCTTTGCAGC-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGCTGCAAAGACAGAGATAGAACTCGAGTTCTATCTCTGTCTTTGCAGCTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGCTGCAAAGACAGAGATAGAACTCGAGTTCTATCTCTGTCTTTGCAGC-3'
Other clones with same target sequence:
(none)
