Construct: shRNA TRCN0000087485

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_130789.3-318s1c1
DNA Barcode:
GCTAAACATGGCCTACAAGTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
1700003N22Rik (229049)
Gene Description:
RIKEN cDNA 1700003N22 gene
Transcript:
RefSeq XM_130789.3 (NON-CURRENT)
Match location:
Position 318 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000087485)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTAAACATGGCCTACAAGTT
Hairpin Sequence:
5'-CCGG-GCTAAACATGGCCTACAAGTT-CTCGAG-AACTTGTAGGCCATGTTTAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTAAACATGGCCTACAAGTTCTCGAGAACTTGTAGGCCATGTTTAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTAAACATGGCCTACAAGTTCTCGAGAACTTGTAGGCCATGTTTAGC-3'

Other clones with same target sequence:

(none)