Construct: shRNA TRCN0000087518

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_135041.5-2510s1c1
DNA Barcode:
GAGGACTGTGAACTGGTTGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Adamts7 (108153)
Gene Description:
a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 7
Transcript:
RefSeq XM_135041.5 (NON-CURRENT)
Match location:
Position 2510 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000087518)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGGACTGTGAACTGGTTGAA
Hairpin Sequence:
5'-CCGG-GAGGACTGTGAACTGGTTGAA-CTCGAG-TTCAACCAGTTCACAGTCCTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGGACTGTGAACTGGTTGAACTCGAGTTCAACCAGTTCACAGTCCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGGACTGTGAACTGGTTGAACTCGAGTTCAACCAGTTCACAGTCCTC-3'

Other clones with same target sequence:

(none)