Construct: shRNA TRCN0000087573

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_356072.2-2386s1c1
DNA Barcode:
CCATTTCCCATGTTCTACCAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
LOC382006 (382006)
Gene Description:
similar to ADAM 25 precursor (A disintegrin and metalloproteinase domain 25) (Testase 2)
Transcript:
RefSeq XM_356072.2 (NON-CURRENT)
Match location:
Position 2386 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000087573)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCATTTCCCATGTTCTACCAT
Hairpin Sequence:
5'-CCGG-CCATTTCCCATGTTCTACCAT-CTCGAG-ATGGTAGAACATGGGAAATGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCATTTCCCATGTTCTACCATCTCGAGATGGTAGAACATGGGAAATGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCATTTCCCATGTTCTACCATCTCGAGATGGTAGAACATGGGAAATGG-3'

Other clones with same target sequence:

(none)