Construct: shRNA TRCN0000087585

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_153397.1-65s1c1
DNA Barcode:
CCCGAGTCTCAAAGTTCATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Adam32 (353188)
Gene Description:
a disintegrin and metallopeptidase domain 32
Transcript:
RefSeq NM_153397.1 (NON-CURRENT)
Match location:
Position 65 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000087585)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCGAGTCTCAAAGTTCATTT
Hairpin Sequence:
5'-CCGG-CCCGAGTCTCAAAGTTCATTT-CTCGAG-AAATGAACTTTGAGACTCGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCGAGTCTCAAAGTTCATTTCTCGAGAAATGAACTTTGAGACTCGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCGAGTCTCAAAGTTCATTTCTCGAGAAATGAACTTTGAGACTCGGG-3'

Other clones with same target sequence:

(none)