Construct: shRNA TRCN0000087602

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009174.2-1255s1c1
DNA Barcode:
CACCACTTCATGCTGGTACTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Siah2 (20439)
Gene Description:
seven in absentia 2
Transcript:
RefSeq NM_009174.2 (NON-CURRENT)
Match location:
Position 1255 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000087602)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CACCACTTCATGCTGGTACTT
Hairpin Sequence:
5'-CCGG-CACCACTTCATGCTGGTACTT-CTCGAG-AAGTACCAGCATGAAGTGGTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCACCACTTCATGCTGGTACTTCTCGAGAAGTACCAGCATGAAGTGGTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACACCACTTCATGCTGGTACTTCTCGAGAAGTACCAGCATGAAGTGGTG-3'

Other clones with same target sequence:

  1. TRCN0000327575