Construct: shRNA TRCN0000087605
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- XM_357786.2-122s1c1
- DNA Barcode:
- AGAAGAAGAAGGTTCCAATAT
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- LOC384679 (384679)
- Gene Description:
- similar to Methionine aminopeptidase 2 (MetAP 2) (Peptidase M 2) (Initiation factor 2 associated 67 kDa glycoprotein) (p67) (p67eIF2)
- Transcript:
- RefSeq XM_357786.2 (NON-CURRENT)
- Match location:
- Position 122 (CDS)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000087605)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- AGAAGAAGAAGGTTCCAATAT
- Hairpin Sequence:
-
5'-CCGG-AGAAGAAGAAGGTTCCAATAT-CTCGAG-ATATTGGAACCTTCTTCTTCT-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGAGAAGAAGAAGGTTCCAATATCTCGAGATATTGGAACCTTCTTCTTCTTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAAGAAGAAGAAGGTTCCAATATCTCGAGATATTGGAACCTTCTTCTTCT-3'
Other clones with same target sequence:
(none)
