Construct: shRNA TRCN0000087629

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_355137.2-1095s1c1
DNA Barcode:
TCGATACGTGATCTACGGAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Naaladl1 (381204)
Gene Description:
N-acetylated alpha-linked acidic dipeptidase-like 1
Transcript:
RefSeq XM_355137.2 (NON-CURRENT)
Match location:
Position 1095 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000087629)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCGATACGTGATCTACGGAAA
Hairpin Sequence:
5'-CCGG-TCGATACGTGATCTACGGAAA-CTCGAG-TTTCCGTAGATCACGTATCGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCGATACGTGATCTACGGAAACTCGAGTTTCCGTAGATCACGTATCGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCGATACGTGATCTACGGAAACTCGAGTTTCCGTAGATCACGTATCGA-3'

Other clones with same target sequence:

(none)