Construct: shRNA TRCN0000087653

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018746.1-2865s1c1
DNA Barcode:
CCCTGCCATGTTGTCCTCGTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Itih4 (16427)
Gene Description:
inter alpha-trypsin inhibitor, heavy chain 4
Transcript:
RefSeq NM_018746.1 (NON-CURRENT)
Match location:
Position 2865 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000087653)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTGCCATGTTGTCCTCGTA
Hairpin Sequence:
5'-CCGG-CCCTGCCATGTTGTCCTCGTA-CTCGAG-TACGAGGACAACATGGCAGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTGCCATGTTGTCCTCGTACTCGAGTACGAGGACAACATGGCAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTGCCATGTTGTCCTCGTACTCGAGTACGAGGACAACATGGCAGGG-3'

Other clones with same target sequence:

(none)