Construct: shRNA TRCN0000087791

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_144907.1-1612s1c1
DNA Barcode:
CGTAGGATGTACAACCTCTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Sesn2 (230784)
Gene Description:
sestrin 2
Transcript:
RefSeq NM_144907.1 (CURRENT)
Match location:
Position 1612 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000087791)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTAGGATGTACAACCTCTTT
Hairpin Sequence:
5'-CCGG-CGTAGGATGTACAACCTCTTT-CTCGAG-AAAGAGGTTGTACATCCTACG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTAGGATGTACAACCTCTTTCTCGAGAAAGAGGTTGTACATCCTACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTAGGATGTACAACCTCTTTCTCGAGAAAGAGGTTGTACATCCTACG-3'

Other clones with same target sequence:

(none)