Construct: shRNA TRCN0000087800

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_289718.2-727s1c1
DNA Barcode:
CCACGCAATTACAAGGTGTCT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ppp1r13l (333654)
Gene Description:
protein phosphatase 1, regulatory (inhibitor) subunit 13 like
Transcript:
RefSeq XM_289718.2 (NON-CURRENT)
Match location:
Position 727 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000087800)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCACGCAATTACAAGGTGTCT
Hairpin Sequence:
5'-CCGG-CCACGCAATTACAAGGTGTCT-CTCGAG-AGACACCTTGTAATTGCGTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCACGCAATTACAAGGTGTCTCTCGAGAGACACCTTGTAATTGCGTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCACGCAATTACAAGGTGTCTCTCGAGAGACACCTTGTAATTGCGTGG-3'

Other clones with same target sequence:

(none)