Construct: shRNA TRCN0000087829

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_358002.2-1351s1c1
DNA Barcode:
CTGGATGACCTTTACCAACAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
LOC385028 (385028)
Gene Description:
similar to HEC protein; highly expressed in cancer, rich in leucine heptad repeats
Transcript:
RefSeq XM_358002.2 (NON-CURRENT)
Match location:
Position 1351 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000087829)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTGGATGACCTTTACCAACAA
Hairpin Sequence:
5'-CCGG-CTGGATGACCTTTACCAACAA-CTCGAG-TTGTTGGTAAAGGTCATCCAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTGGATGACCTTTACCAACAACTCGAGTTGTTGGTAAAGGTCATCCAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTGGATGACCTTTACCAACAACTCGAGTTGTTGGTAAAGGTCATCCAG-3'

Other clones with same target sequence:

(none)