Construct: shRNA TRCN0000087903

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_137276.6-1674s1c1
DNA Barcode:
GCCTCCAGAAATGAACCCTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gas2l3 (237436)
Gene Description:
growth arrest-specific 2 like 3
Transcript:
RefSeq XM_137276.6 (NON-CURRENT)
Match location:
Position 1674 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000087903)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTCCAGAAATGAACCCTTT
Hairpin Sequence:
5'-CCGG-GCCTCCAGAAATGAACCCTTT-CTCGAG-AAAGGGTTCATTTCTGGAGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTCCAGAAATGAACCCTTTCTCGAGAAAGGGTTCATTTCTGGAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTCCAGAAATGAACCCTTTCTCGAGAAAGGGTTCATTTCTGGAGGC-3'

Other clones with same target sequence:

(none)