Construct: shRNA TRCN0000087927

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172301.2-683s1c1
DNA Barcode:
CTATCCTCATTGACTGGCTAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ccnb1 (268697)
Gene Description:
cyclin B1
Transcript:
RefSeq NM_172301.2 (NON-CURRENT)
Match location:
Position 683 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000087927)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTATCCTCATTGACTGGCTAA
Hairpin Sequence:
5'-CCGG-CTATCCTCATTGACTGGCTAA-CTCGAG-TTAGCCAGTCAATGAGGATAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTATCCTCATTGACTGGCTAACTCGAGTTAGCCAGTCAATGAGGATAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTATCCTCATTGACTGGCTAACTCGAGTTAGCCAGTCAATGAGGATAG-3'

Other clones with same target sequence:

  1. TRCN0000332715