Construct: shRNA TRCN0000087932

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_485561.2-241s1c1
DNA Barcode:
CAGAACAATACTGAGTCCTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gm5288 (384144)
Gene Description:
predicted gene 5288; anaphase-promoting complex subunit 5 pseudogene
Transcript:
RefSeq XM_485561.2 (NON-CURRENT)
Match location:
Position 241 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000087932)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGAACAATACTGAGTCCTTT
Hairpin Sequence:
5'-CCGG-CAGAACAATACTGAGTCCTTT-CTCGAG-AAAGGACTCAGTATTGTTCTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGAACAATACTGAGTCCTTTCTCGAGAAAGGACTCAGTATTGTTCTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGAACAATACTGAGTCCTTTCTCGAGAAAGGACTCAGTATTGTTCTG-3'

Other clones with same target sequence:

(none)