Construct: shRNA TRCN0000087977

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_023503.2-649s1c1
DNA Barcode:
CCAAGAAACGTTAAAGGAAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ing2 (69260)
Gene Description:
inhibitor of growth family, member 2
Transcript:
RefSeq NM_023503.2 (NON-CURRENT)
Match location:
Position 649 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000087977)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAAGAAACGTTAAAGGAAAT
Hairpin Sequence:
5'-CCGG-CCAAGAAACGTTAAAGGAAAT-CTCGAG-ATTTCCTTTAACGTTTCTTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAAGAAACGTTAAAGGAAATCTCGAGATTTCCTTTAACGTTTCTTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAAGAAACGTTAAAGGAAATCTCGAGATTTCCTTTAACGTTTCTTGG-3'

Other clones with same target sequence:

(none)