Construct: shRNA TRCN0000087989

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025482.2-587s1c1
DNA Barcode:
GCCACCTTCAAGTCATTTGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tpd52l2 (66314)
Gene Description:
tumor protein D52-like 2
Transcript:
RefSeq NM_025482.2 (NON-CURRENT)
Match location:
Position 587 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000087989)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCACCTTCAAGTCATTTGAA
Hairpin Sequence:
5'-CCGG-GCCACCTTCAAGTCATTTGAA-CTCGAG-TTCAAATGACTTGAAGGTGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCACCTTCAAGTCATTTGAACTCGAGTTCAAATGACTTGAAGGTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCACCTTCAAGTCATTTGAACTCGAGTTCAAATGACTTGAAGGTGGC-3'

Other clones with same target sequence:

  1. TRCN0000312146