Construct: shRNA TRCN0000087995

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_485560.2-1484s1c1
DNA Barcode:
GCACTGTTTGAGCTGGCTTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gm10482 (433844)
Gene Description:
predicted gene 10482
Transcript:
RefSeq XM_485560.2 (NON-CURRENT)
Match location:
Position 1484 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000087995)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCACTGTTTGAGCTGGCTTTA
Hairpin Sequence:
5'-CCGG-GCACTGTTTGAGCTGGCTTTA-CTCGAG-TAAAGCCAGCTCAAACAGTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCACTGTTTGAGCTGGCTTTACTCGAGTAAAGCCAGCTCAAACAGTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCACTGTTTGAGCTGGCTTTACTCGAGTAAAGCCAGCTCAAACAGTGC-3'

Other clones with same target sequence:

(none)