Construct: shRNA TRCN0000088005

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_485588.1-370s1c1
DNA Barcode:
CCATCTACTAAAGATTTCCTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
LOC433869 (433869)
Gene Description:
similar to HEC
Transcript:
RefSeq XM_485588.1 (NON-CURRENT)
Match location:
Position 370 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000088005)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCATCTACTAAAGATTTCCTA
Hairpin Sequence:
5'-CCGG-CCATCTACTAAAGATTTCCTA-CTCGAG-TAGGAAATCTTTAGTAGATGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCATCTACTAAAGATTTCCTACTCGAGTAGGAAATCTTTAGTAGATGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCATCTACTAAAGATTTCCTACTCGAGTAGGAAATCTTTAGTAGATGG-3'

Other clones with same target sequence:

(none)