Construct: shRNA TRCN0000088055

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_283394.2-212s1c1
DNA Barcode:
TCTTGGAGACATTGGTAATAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ccnb1-rs5 (328752)
Gene Description:
cyclin B1, related sequence 5
Transcript:
RefSeq XM_283394.2 (NON-CURRENT)
Match location:
Position 212 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000088055)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCTTGGAGACATTGGTAATAA
Hairpin Sequence:
5'-CCGG-TCTTGGAGACATTGGTAATAA-CTCGAG-TTATTACCAATGTCTCCAAGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCTTGGAGACATTGGTAATAACTCGAGTTATTACCAATGTCTCCAAGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCTTGGAGACATTGGTAATAACTCGAGTTATTACCAATGTCTCCAAGA-3'

Other clones with same target sequence:

(none)