Construct: shRNA TRCN0000088100

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_355347.3-1409s1c1
DNA Barcode:
CCGAGCCAATCCAGTATCATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
BC053994 (381383)
Gene Description:
cDNA sequence BC053994
Transcript:
RefSeq XM_355347.3 (NON-CURRENT)
Match location:
Position 1409 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000088100)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGAGCCAATCCAGTATCATT
Hairpin Sequence:
5'-CCGG-CCGAGCCAATCCAGTATCATT-CTCGAG-AATGATACTGGATTGGCTCGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGAGCCAATCCAGTATCATTCTCGAGAATGATACTGGATTGGCTCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGAGCCAATCCAGTATCATTCTCGAGAATGATACTGGATTGGCTCGG-3'

Other clones with same target sequence:

(none)