Construct: shRNA TRCN0000088198

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_173378.1-3723s1c1
DNA Barcode:
GCAGTAACTATTGTATGAGTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Trp53bp2 (209456)
Gene Description:
transformation related protein 53 binding protein 2
Transcript:
RefSeq NM_173378.1 (NON-CURRENT)
Match location:
Position 3723 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000088198)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGTAACTATTGTATGAGTT
Hairpin Sequence:
5'-CCGG-GCAGTAACTATTGTATGAGTT-CTCGAG-AACTCATACAATAGTTACTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGTAACTATTGTATGAGTTCTCGAGAACTCATACAATAGTTACTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGTAACTATTGTATGAGTTCTCGAGAACTCATACAATAGTTACTGC-3'

Other clones with same target sequence:

(none)