Construct: shRNA TRCN0000088199

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_173378.1-3216s1c1
DNA Barcode:
GCTAGGACTATACCCAAGAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Trp53bp2 (209456)
Gene Description:
transformation related protein 53 binding protein 2
Transcript:
RefSeq NM_173378.1 (NON-CURRENT)
Match location:
Position 3216 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000088199)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTAGGACTATACCCAAGAAT
Hairpin Sequence:
5'-CCGG-GCTAGGACTATACCCAAGAAT-CTCGAG-ATTCTTGGGTATAGTCCTAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTAGGACTATACCCAAGAATCTCGAGATTCTTGGGTATAGTCCTAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTAGGACTATACCCAAGAATCTCGAGATTCTTGGGTATAGTCCTAGC-3'

Other clones with same target sequence:

(none)