Construct: shRNA TRCN0000088271

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_137762.3-388s1c1
DNA Barcode:
GCCATCTATCACCTCAGAGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
1700071K01Rik (237880)
Gene Description:
RIKEN cDNA 1700071K01 gene
Transcript:
RefSeq XM_137762.3 (NON-CURRENT)
Match location:
Position 388 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000088271)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCATCTATCACCTCAGAGAT
Hairpin Sequence:
5'-CCGG-GCCATCTATCACCTCAGAGAT-CTCGAG-ATCTCTGAGGTGATAGATGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCATCTATCACCTCAGAGATCTCGAGATCTCTGAGGTGATAGATGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCATCTATCACCTCAGAGATCTCGAGATCTCTGAGGTGATAGATGGC-3'

Other clones with same target sequence:

(none)