Construct: shRNA TRCN0000088495

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172516.3-775s1c1
DNA Barcode:
CCTGTGATAACCTATGCACTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Dstyk (213452)
Gene Description:
dual serine/threonine and tyrosine protein kinase
Transcript:
RefSeq NM_172516.3 (NON-CURRENT)
Match location:
Position 775 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000088495)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGTGATAACCTATGCACTT
Hairpin Sequence:
5'-CCGG-CCTGTGATAACCTATGCACTT-CTCGAG-AAGTGCATAGGTTATCACAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGTGATAACCTATGCACTTCTCGAGAAGTGCATAGGTTATCACAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGTGATAACCTATGCACTTCTCGAGAAGTGCATAGGTTATCACAGG-3'

Other clones with same target sequence:

  1. TRCN0000288104