Construct: shRNA TRCN0000088514

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_027230.2-1009s1c1
DNA Barcode:
GCCCGTTTCTTTGGACAACAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Zmynd8 (228880)
Gene Description:
zinc finger, MYND-type containing 8
Transcript:
RefSeq NM_027230.2 (NON-CURRENT)
Match location:
Position 1009 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000088514)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCCGTTTCTTTGGACAACAT
Hairpin Sequence:
5'-CCGG-GCCCGTTTCTTTGGACAACAT-CTCGAG-ATGTTGTCCAAAGAAACGGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCCGTTTCTTTGGACAACATCTCGAGATGTTGTCCAAAGAAACGGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCCGTTTCTTTGGACAACATCTCGAGATGTTGTCCAAAGAAACGGGC-3'

Other clones with same target sequence:

(none)