Construct: shRNA TRCN0000088521

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145076.1-1837s1c1
DNA Barcode:
CCACCACGCTTAATAAACTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Trim24 (21848)
Gene Description:
tripartite motif-containing 24
Transcript:
RefSeq NM_145076.1 (NON-CURRENT)
Match location:
Position 1837 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000088521)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCACCACGCTTAATAAACTTT
Hairpin Sequence:
5'-CCGG-CCACCACGCTTAATAAACTTT-CTCGAG-AAAGTTTATTAAGCGTGGTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCACCACGCTTAATAAACTTTCTCGAGAAAGTTTATTAAGCGTGGTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCACCACGCTTAATAAACTTTCTCGAGAAAGTTTATTAAGCGTGGTGG-3'

Other clones with same target sequence:

  1. TRCN0000288111