Construct: shRNA TRCN0000088586

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_133853.1-1120s1c1
DNA Barcode:
CCCTCAGTATGGAACATACTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Magi3 (99470)
Gene Description:
membrane associated guanylate kinase, WW and PDZ domain containing 3
Transcript:
RefSeq NM_133853.1 (NON-CURRENT)
Match location:
Position 1120 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000088586)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTCAGTATGGAACATACTA
Hairpin Sequence:
5'-CCGG-CCCTCAGTATGGAACATACTA-CTCGAG-TAGTATGTTCCATACTGAGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTCAGTATGGAACATACTACTCGAGTAGTATGTTCCATACTGAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTCAGTATGGAACATACTACTCGAGTAGTATGTTCCATACTGAGGG-3'

Other clones with same target sequence:

(none)