Construct: shRNA TRCN0000088591

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_194462.1-6865s1c1
DNA Barcode:
CGGTTAAATGAGCAGTTTATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Akap9 (100986)
Gene Description:
A kinase (PRKA) anchor protein (yotiao) 9
Transcript:
RefSeq NM_194462.1 (NON-CURRENT)
Match location:
Position 6865 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000088591)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGTTAAATGAGCAGTTTATA
Hairpin Sequence:
5'-CCGG-CGGTTAAATGAGCAGTTTATA-CTCGAG-TATAAACTGCTCATTTAACCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGTTAAATGAGCAGTTTATACTCGAGTATAAACTGCTCATTTAACCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGTTAAATGAGCAGTTTATACTCGAGTATAAACTGCTCATTTAACCG-3'

Other clones with same target sequence:

(none)