Construct: shRNA TRCN0000088603

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011106.1-638s1c1
DNA Barcode:
GCACCATGAGATGTTATTTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pkig (18769)
Gene Description:
protein kinase inhibitor, gamma
Transcript:
RefSeq NM_011106.1 (NON-CURRENT)
Match location:
Position 638 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000088603)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCACCATGAGATGTTATTTAT
Hairpin Sequence:
5'-CCGG-GCACCATGAGATGTTATTTAT-CTCGAG-ATAAATAACATCTCATGGTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCACCATGAGATGTTATTTATCTCGAGATAAATAACATCTCATGGTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCACCATGAGATGTTATTTATCTCGAGATAAATAACATCTCATGGTGC-3'

Other clones with same target sequence:

(none)