Construct: shRNA TRCN0000088609

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_177320.1-1233s1c1
DNA Barcode:
GCAGAAGTTCAACAGGTTCTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pik3r5 (320207)
Gene Description:
phosphoinositide-3-kinase regulatory subunit 5
Transcript:
RefSeq NM_177320.1 (NON-CURRENT)
Match location:
Position 1233 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000088609)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGAAGTTCAACAGGTTCTA
Hairpin Sequence:
5'-CCGG-GCAGAAGTTCAACAGGTTCTA-CTCGAG-TAGAACCTGTTGAACTTCTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGAAGTTCAACAGGTTCTACTCGAGTAGAACCTGTTGAACTTCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGAAGTTCAACAGGTTCTACTCGAGTAGAACCTGTTGAACTTCTGC-3'

Other clones with same target sequence:

(none)