Construct: shRNA TRCN0000088644

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_028994.1-1799s1c1
DNA Barcode:
CCTCGTATCTTCCATGTCAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pck2 (74551)
Gene Description:
phosphoenolpyruvate carboxykinase 2 (mitochondrial)
Transcript:
RefSeq NM_028994.1 (NON-CURRENT)
Match location:
Position 1799 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000088644)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCGTATCTTCCATGTCAAT
Hairpin Sequence:
5'-CCGG-CCTCGTATCTTCCATGTCAAT-CTCGAG-ATTGACATGGAAGATACGAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCGTATCTTCCATGTCAATCTCGAGATTGACATGGAAGATACGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCGTATCTTCCATGTCAATCTCGAGATTGACATGGAAGATACGAGG-3'

Other clones with same target sequence:

  1. TRCN0000363752