Construct: shRNA TRCN0000088648

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021921.2-2969s1c1
DNA Barcode:
GCCCTTCTCTTGTGATTTATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mapk8ip2 (60597)
Gene Description:
mitogen-activated protein kinase 8 interacting protein 2
Transcript:
RefSeq NM_021921.2 (NON-CURRENT)
Match location:
Position 2969 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000088648)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCCTTCTCTTGTGATTTATA
Hairpin Sequence:
5'-CCGG-GCCCTTCTCTTGTGATTTATA-CTCGAG-TATAAATCACAAGAGAAGGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCCTTCTCTTGTGATTTATACTCGAGTATAAATCACAAGAGAAGGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCCTTCTCTTGTGATTTATACTCGAGTATAAATCACAAGAGAAGGGC-3'

Other clones with same target sequence:

(none)