Construct: shRNA TRCN0000088684

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_620559.1-5903s1c1
DNA Barcode:
CCTCATATTATATCCTGCAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Smg1 (233789)
Gene Description:
SMG1 homolog, phosphatidylinositol 3-kinase-related kinase (C. elegans)
Transcript:
RefSeq XM_620559.1 (NON-CURRENT)
Match location:
Position 5903 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000088684)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCATATTATATCCTGCAAT
Hairpin Sequence:
5'-CCGG-CCTCATATTATATCCTGCAAT-CTCGAG-ATTGCAGGATATAATATGAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCATATTATATCCTGCAATCTCGAGATTGCAGGATATAATATGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCATATTATATCCTGCAATCTCGAGATTGCAGGATATAATATGAGG-3'

Other clones with same target sequence:

(none)