Construct: shRNA TRCN0000088716

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_013931.1-427s1c1
DNA Barcode:
CCGAGGAGAAATTCATCGAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mapk8ip3 (30957)
Gene Description:
mitogen-activated protein kinase 8 interacting protein 3
Transcript:
RefSeq NM_013931.1 (NON-CURRENT)
Match location:
Position 427 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000088716)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGAGGAGAAATTCATCGAAT
Hairpin Sequence:
5'-CCGG-CCGAGGAGAAATTCATCGAAT-CTCGAG-ATTCGATGAATTTCTCCTCGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGAGGAGAAATTCATCGAATCTCGAGATTCGATGAATTTCTCCTCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGAGGAGAAATTCATCGAATCTCGAGATTCGATGAATTTCTCCTCGG-3'

Other clones with same target sequence:

(none)