Construct: shRNA TRCN0000088722

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145571.1-679s1c1
DNA Barcode:
TCTTCAGGGTTTATGCCCATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mob1a (232157)
Gene Description:
MOB kinase activator 1A
Transcript:
RefSeq NM_145571.1 (NON-CURRENT)
Match location:
Position 679 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000088722)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCTTCAGGGTTTATGCCCATA
Hairpin Sequence:
5'-CCGG-TCTTCAGGGTTTATGCCCATA-CTCGAG-TATGGGCATAAACCCTGAAGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCTTCAGGGTTTATGCCCATACTCGAGTATGGGCATAAACCCTGAAGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCTTCAGGGTTTATGCCCATACTCGAGTATGGGCATAAACCCTGAAGA-3'

Other clones with same target sequence:

  1. TRCN0000324301