Construct: shRNA TRCN0000088744

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_619763.1-6735s1c1
DNA Barcode:
GCCCAGTAACAAATATGAAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
LOC545424 (545424)
Gene Description:
similar to cardiac titin N2BA isoform
Transcript:
RefSeq XM_619763.1 (NON-CURRENT)
Match location:
Position 6735 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000088744)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCCAGTAACAAATATGAAAT
Hairpin Sequence:
5'-CCGG-GCCCAGTAACAAATATGAAAT-CTCGAG-ATTTCATATTTGTTACTGGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCCAGTAACAAATATGAAATCTCGAGATTTCATATTTGTTACTGGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCCAGTAACAAATATGAAATCTCGAGATTTCATATTTGTTACTGGGC-3'

Other clones with same target sequence:

(none)