Construct: shRNA TRCN0000088770

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019774.2-1317s1c1
DNA Barcode:
GCCCGACAAGACAGTAGAATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Akap8 (56399)
Gene Description:
A kinase (PRKA) anchor protein 8
Transcript:
RefSeq NM_019774.2 (NON-CURRENT)
Match location:
Position 1317 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000088770)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCCGACAAGACAGTAGAATT
Hairpin Sequence:
5'-CCGG-GCCCGACAAGACAGTAGAATT-CTCGAG-AATTCTACTGTCTTGTCGGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCCGACAAGACAGTAGAATTCTCGAGAATTCTACTGTCTTGTCGGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCCGACAAGACAGTAGAATTCTCGAGAATTCTACTGTCTTGTCGGGC-3'

Other clones with same target sequence:

(none)