Construct: shRNA TRCN0000088786

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178061.3-918s1c1
DNA Barcode:
TCATCACTTTGACCGTGTCAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mob3b (214944)
Gene Description:
MOB kinase activator 3B
Transcript:
RefSeq NM_178061.3 (NON-CURRENT)
Match location:
Position 918 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000088786)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCATCACTTTGACCGTGTCAT
Hairpin Sequence:
5'-CCGG-TCATCACTTTGACCGTGTCAT-CTCGAG-ATGACACGGTCAAAGTGATGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCATCACTTTGACCGTGTCATCTCGAGATGACACGGTCAAAGTGATGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCATCACTTTGACCGTGTCATCTCGAGATGACACGGTCAAAGTGATGA-3'

Other clones with same target sequence:

(none)