Construct: shRNA TRCN0000088830

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_485946.1-4424s1c1
DNA Barcode:
CAAGGAGCATATCAACATCAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
LOC434196 (434196)
Gene Description:
similar to mKIAA1790 protein
Transcript:
RefSeq XM_485946.1 (NON-CURRENT)
Match location:
Position 4424 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000088830)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAAGGAGCATATCAACATCAA
Hairpin Sequence:
5'-CCGG-CAAGGAGCATATCAACATCAA-CTCGAG-TTGATGTTGATATGCTCCTTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAAGGAGCATATCAACATCAACTCGAGTTGATGTTGATATGCTCCTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAAGGAGCATATCAACATCAACTCGAGTTGATGTTGATATGCTCCTTG-3'

Other clones with same target sequence:

(none)