Construct: shRNA TRCN0000088834

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011499.1-458s1c1
DNA Barcode:
CGCATATATGACTTGAACAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Strap (20901)
Gene Description:
serine/threonine kinase receptor associated protein
Transcript:
RefSeq NM_011499.1 (NON-CURRENT)
Match location:
Position 458 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000088834)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGCATATATGACTTGAACAAA
Hairpin Sequence:
5'-CCGG-CGCATATATGACTTGAACAAA-CTCGAG-TTTGTTCAAGTCATATATGCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCATATATGACTTGAACAAACTCGAGTTTGTTCAAGTCATATATGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCATATATGACTTGAACAAACTCGAGTTTGTTCAAGTCATATATGCG-3'

Other clones with same target sequence:

  1. TRCN0000324156