Construct: shRNA TRCN0000088898

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_028903.1-2265s1c1
DNA Barcode:
CCCAGCAAGTAGAAACCCTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Scara5 (71145)
Gene Description:
scavenger receptor class A, member 5
Transcript:
RefSeq NM_028903.1 (NON-CURRENT)
Match location:
Position 2265 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000088898)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCAGCAAGTAGAAACCCTTA
Hairpin Sequence:
5'-CCGG-CCCAGCAAGTAGAAACCCTTA-CTCGAG-TAAGGGTTTCTACTTGCTGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCAGCAAGTAGAAACCCTTACTCGAGTAAGGGTTTCTACTTGCTGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCAGCAAGTAGAAACCCTTACTCGAGTAAGGGTTTCTACTTGCTGGG-3'

Other clones with same target sequence:

  1. TRCN0000335628