Construct: shRNA TRCN0000089093

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008073.1-1932s1c1
DNA Barcode:
CCGTGGTTATTGTAATTTGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gabrg2 (14406)
Gene Description:
gamma-aminobutyric acid (GABA) A receptor, subunit gamma 2
Transcript:
RefSeq NM_008073.1 (NON-CURRENT)
Match location:
Position 1932 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000089093)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGTGGTTATTGTAATTTGAA
Hairpin Sequence:
5'-CCGG-CCGTGGTTATTGTAATTTGAA-CTCGAG-TTCAAATTACAATAACCACGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGTGGTTATTGTAATTTGAACTCGAGTTCAAATTACAATAACCACGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGTGGTTATTGTAATTTGAACTCGAGTTCAAATTACAATAACCACGG-3'

Other clones with same target sequence:

(none)